Spectral Applied Imaging

Spectral Applied Research Microscope Automation

LMM5 Laser Merge Module

Many modern instruments can utilize several laser wavelengths for illumination. The LMM5 sets a new standard for laser merge modules. The LMM5 couples the light from 1 to 5 solid state lasers into a single fiber optic cable. The light from the fiber can be used to drive a number of devices but the LMM5 was designed with confocal and TIRF microscopy in mind. The LMM5 has the performance and control characteristics that make it ideally suited to the requirements of modern live cell imaging.

The LMM5 uses shutters for positive on/off control with rapid switching time. A continuously variable ND (neutral density) filter wheel allows for individual laser power control to optimize exposures. All controls are through a USB port although the shutters have a manual override on the control unit.

Spectral Applied Research LMM5 Laser Merge Module

Spectral Applied Research LMM5 Laser Merge Module

A flexible mounting system allows most diode and DPSS (diode pumped solid state) lasers to be easily mounted. Although 5 lasers can be mounted, the system can be sold with fewer lasers and the customer can add additional lasers at a later date. The most common laser wavelengths are 405nm, 440nm, 488nm, 491nm, 561nm, and 655nm.

The small footprint and novel fiber exit geometry allow the unit to be placed on or under the table while taking little valuable lab space. The robust design means that the unit can be moved, bumped or have things stacked on top of it without affecting the alignment.

Spectral has developed a module that can combine the outputs of 2‒5 diode lasers into a single fibre optic cable for input into the instrument. This equipment is intended to use Class IIIb lasers so that the Laser Merge Module is classified as a Class IIIb laser product. The LMM5 is designed to meet the requirements of European standard IEC 60825-1:1993+A1:1997+A2:2001 and US standard 21 CFR 1040.10 and 1040.11 except for deviations pursuant to Laser Notice No. 50, dated July 26, 2001.

Features

  • Choose from a wide selection of available lasers
  • Rear fiber exit allows for table top or floor mounting
  • Compatible with single mode, polarization maintaining, or multimode fiber
  • Low heat load and low input power requirements
  • USB, RS‐232, or TTL interfaces for computer control
  • High coupling efficiency
  • Independent laser power control from 1 to 100%
  • Fast mechanical shutters provide positive laser selection and exposure control
  • Fiber‐coupled power monitor for eye safe power measurement
  • Master key switch, remote interlock and emission indicators for safety
  • Optional acousto-optic tunable filter (AOTF) to replace shutters and ND filters

Specifications

General
Operating Range: 400‒700 nm
Numerical Aperture: 0.08‒0.12
Coupling Efficiency: 60‒80%
Shutter
Exposure Time: 1.5 ms or greater
Repetition Rate: 50 Hz
Repetition Rate (Burst): 200 Hz
ND Filter Wheel
Attenuation: 0‒99%
Maximum Time to Scan Full Range: 10 seconds
AOTF
Transmission: 0‒80%
Switching Time: 20 μsec
Physical
Dimensions: 21.5×10.5×13″ (55×27×34 cm)
Weight: ~25kg with 2 lasers; ~35kg with 5 lasers
Electrical
Voltage requirements: 120/240V, 50‒60 Hz

Wavelength Selection

The following table represents the wavelengths and power levels that are currently available for the LMM5. New wavelengths and power levels are likely to be available in the near future. All lasers are Class IIIb units.

Wavelength (nm) Laser Power Levels (mW)
405 20, 25, 30, 35, 50, 55, 110
408 48
440 15, 16, 20, 40
460 10
473 4, 10, 15, 20, 25, 50
488 10, 20, 30, 40, 100, 200
491 25, 50
491‒532 20
523 20
532 20, 25, 30, 50, 75, 100
542 20
561 15, 20
635 25
638 24
640 12
650 60, 200
655 20‒30, 25
658 24, 30
660 25
685 25